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MagMAX™-96 AI/ND Viral RNA Isolation Kit

 

Catalog number:  AM1835

Description:

Applied Biosystems® This kit is for the isolation of viral RNA and DNA from diverse sample types (i.e., environmental samples and biofluids). Nucleic acids purified using this kit are ideal for PCR and RT-PCR applications. This is a first generation MagMAX™ Viral RNA Isolation Kit validated by the NVSL for use with AI/ND viruses. Perfect for working with low viral concentrations, the kit contains sufficient reagents for 4 x 96 reactions.
 

  • Validated by the NVSL for use with AI/ND viruses
  • Recover as few as 10 copies of viral RNA from 100–400 µL sample
  • Typical viral RNA recovery of >50%
  • Ideal when working with low viral concentrations
  • Linear recovery down to 50 transcripts
  • 96 samples can be processed in 30 minutes
  • Easily integrated into robotic platforms


Benefits of MagMAX™ Magnetic Bead-Based Purification:
Magnetic beads offer many benefits compared to other technologies for isolating RNA. Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples. Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20–50 µL of nuclease-free water, concentrating RNA from large, dilute samples.

Optimized for Isolation of Virus from Biofluids:
The Applied Biosystems® MagMAX™ Viral RNA Isolation Kit has been optimized for use with biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media. These samples often present unique challenges when purifying RNA, including low viral titers, large volumes to be processed, and sample complexity. Without the need for organic solvents or RNA precipitation, the MagMAX™ Viral RNA isolation kit eliminates the problems often encountered with other RNA isolation methods such filter clogging, large elution volumes, and inconsistent yields.

Specifications:

Sample Type (General):

Liquid Samples (e.g. Serum), Viral Samples

Sample Type (Specific):

Any Swab Type, Cell-Free Fluids, Plasma, Serum

Final Product:

Viral RNA

Downstream Application:

Northern Blotting, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)

For Use With (Equipment):

KingFisher™ Flex Magnetic Particle Processor, MagMAX™ Express-96 Standard Magnetic Particle Processor

High Throughput Compatibility:

Automated Protocols, High Throughput-Compatible, Not High Throughput-Compatible (Manual)

Isolation Technology:

Magnetic Bead

Format:

96-well plate

Number of Reactions:

384 Reactions

Product Line:

MagMAX™

Product Size:

384 preps

Purification Time:

1 hrs

Starting Material (Amount):

Up to 50µl of sample

Yield (up to):

5 ng